亚洲视频一区二区三区四区-亚洲视频一区二区三区-亚洲视频一区二区-亚洲视频一区-亚洲视频一二三-亚洲视频一二区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1589 IEC-18 大鼠回腸細(xì)胞

CRL-1589 IEC-18 大鼠回腸細(xì)胞

簡要描述:CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:1915

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

CRL-1589 IEC-18 大鼠回腸細(xì)胞 的詳細(xì)介紹


ATCC® Number:CRL-1589™  Price:$329.00
Designations:IEC-18

Depositors:A Quaroni

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: ileum
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 嫩草视频在线观看视频播放| 国产亚洲sss在线观看| 精品欧美一区二区三区在线观看| jj免费视频| 白丝美女同人18漫画| 把老师操了| 久久久精品免费视频| 国产精品视频免费看| 果冻传媒在线播放观看228集 | 男人亚洲天堂| 午夜影院免费看| 欧美一区二区三区四区五区六区| 欧美日韩一品道| 性肥胖BWBWBW| 翁息肉小说老扒| 妇乱子伦激情| 国产成人福利免费观看| 成人在线一区二区| 99视频在线国产| 欧美最新在线| 日韩欧美色| 日本免费在线观看| 污黄在线观看| 狐媚小说| 成人免费视频播放| 国产三及| 青山葵在线| 亚洲欧洲日产国码天堂| 91制片在线观看| 国产一卡二卡3卡4卡四卡在线| 大学生按摩黄a级中文片| 国产精品免费aⅴ片在线观看| 日本一卡二卡3卡四卡网站精品 | 阿v天堂2020| 亚洲欧美国产在线| 日本色淫| 成年无限观看onlyfans| 14一15sexvideo日本| 高清欧美不卡一区二区三区| 亚欧毛片基地国产毛片基地| 女同志 videos|