亚洲视频一区二区三区四区-亚洲视频一区二区三区-亚洲视频一区二区-亚洲视频一区-亚洲视频一二三-亚洲视频一二区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2547Panc 10.05 人胰腺腺癌細(xì)胞

Panc 10.05 人胰腺腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2547
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問  量:1773

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞

ATCC® Number: CRL-2547™ Price: $338.00

Designations: Panc 10.05

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2547 Panc 10.05 人胰腺腺癌細(xì)胞

Isolation: Isolation date: 1992

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II -

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12

D16S539: 9,12

D5S818: 13

D7S820: 8,9

THO1: 6,9.3

TPOX: 11

vWA: 16

Age: adult

Gender: male

Ethnicity: White

Comments: Panc 10.05 is a pancreatic adenocarcinoma epithelial cell line derived in 1992 from a primary tumor removed from the head-of-the-pancreas of a male with pancreatic adenocarcinoma.

Both the PL45 and the Panc 10.05 cell lines exhibit a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 40%. [50655]

The Panc 10.05 cell line was derived from the same patient as the PL45 cell line (ATCC CRL-2558).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 19.2 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derived from same individual:ATCC CRL-2558

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602



CRL-2547
來自ATCC細(xì)胞,所有出入庫細(xì)胞均經(jīng)過嚴(yán)格的細(xì)胞質(zhì)量檢測(cè),確保細(xì)胞無污染,符合檢測(cè)合格標(biāo)準(zhǔn)。歡迎來詢價(jià)詳談


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 色橹橹| 无人在线观看免费高清视频播放| bbox撕裂bass孕妇| 99在线在线视频免费视频观看| 免费观看网站| 99在线免费观看| 99视频导航| 亚洲精品免费在线观看| 奇米7777第四色| 激情小说欧美图片| 狠狠澡| 日韩中文在线| 美女自插| 亚洲精品 欧美| 日本高清在线播放一区二区三区| 厨房里摸着乳丰满在线观看| 亚洲成色www久久网站| 蜜桃破解版免费看nba| 四虎成人免费观看在线网址| 亚洲精品在线免费| 亚洲国产成人在线视频| 放荡女小洁的性日记| 岛国片免费看| jzz大全部| m3u8久久国产精品影院| 9re视频这里只有精品| 99re在线视频观看| 国产成人成人一区二区| 古装床戏做爰无遮挡三级| 久草青青在线| 99ri国产精品| 动漫美女强行被吸乳做羞羞事 | 好大好深视频| 久久久久久久久人体| 99久久精品免费看国产| 九九99香蕉在线视频美国毛片| 91亚色视频在线观看| 狠狠五月天中文字幕| 草草视频人人爽| 齐天大性之七仙女欲春迅雷链接| 国产激情影院|