亚洲视频一区二区三区四区-亚洲视频一区二区三区-亚洲视频一区二区-亚洲视频一区-亚洲视频一二三-亚洲视频一二区

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

簡要描述:CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-15
  • 訪  問  量:2965

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞

ATCC® Number:  CL-188™

Designations:  LS 174T

Depositors:   Northwestern University

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: colon

Tumor Stage: Dukes' type B

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) (The production of CEA in the ATCC seed stock was 1944 ng per 10(6) cells in 10 days.)

interleukin 10 (IL-10) [22511]

interleukin 6 (IL-6)

mucin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Tumorigenic: Yes

Reverse Transcript: negative

Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src -

Antigen Expression: serologically defined colon cancer antigen 3; Homo sapiens, expressed

HLA A2, B13, B50; Blood type O

Cytogenetic Analysis: 45,X; one X chromosome missing; no other chromosomal aberrations

Isoenzymes:  ADA, 1

ES-D, 1

G6PD, B

PEP-D, 1

PGD, A

PGM1, 1

PGM3, 2

Age:  58 years

Gender:  female

Ethnicity:  Caucasian

Comments: LS 174T is a variant of LS 180 (ATCC CL-187) that has been maintained by using trypsin in the subculture protocol. It is more easily subc*ted than that parent line and, like LS 180, it is reported to produce large amounts of carcinoembryonic antigen (CEA).

Electron microscopic studies revealed abundant microvilli and intracytoplasmic mucin vacuoles [Pubmed ID: 1262041].

They are negative for p53 antigen expression, but positive for mRNA expression.

LS 174T cells stain positively for cytokeratins.

The line is positive for expression of c-myc, N-myc, H-ras, N-ras.

Myb, and fos oncogenes.

K-ras and sis oncogene expression were not detected.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

derived from same individual:ATCC CL-187

References: 2148: Tom BH, et al. Human colonic adenocarcinoma cells. I. Establishment and description of a new line. In Vitro 12: 180-191, 1976. PubMed: 1262041

3524: Tom BH, et al. Process of producing carcinoembryonic antigen. US Patent 4,228,236 dated Oct 14 1980

22147: Chen TR, et al. Intercellular karyotypic similarity in near-diploid cell lines of human tumor origins. Cancer Genet. Cytogenet. 10: 351-362, 1983. PubMed: 6652615

22511: Gastl GA, et al. Interleukin-10 production by human carcinoma cell lines and its relationship to interleukin-6 expression. Int. J. Cancer 55: 96-101, 1993. PubMed: 8344757

22861: Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

32265: Tsao H, et al. Novel mutations in the p16/CDKN2A binding region of the Cyclin-dependent Kinase-4 gene. Cancer Res. 58: 109-113, 1998. PubMed: 9426066

32377: Shibata D, et al. Genomic instability in repeated sequences is an early somatic event in colorectal tumorigenesis that persists after transformation. Nat. Genet. 6: 273-281, 1994. PubMed: 8012390

32794: Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

CRL-188 LS 174T 人結(jié)腸腺癌細(xì)胞



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 精品国产福利在线| 精品日本一区二区| 亚洲国产99在线精品一区二区| 欧美日韩综合网在线观看| www.av免费| 男人肌肌捅女人肌肌| 亚洲精彩视频在线观看| 国产高清不卡码一区二区三区| 2019国内精品久久久久久| 天天色天天舔| 金牛网155755水心论坛黄大父母| china外卖员gay国产xnxx| 午夜影院和视费x看| 女人张开腿让男人桶视频免费大全| 高中生放荡日记高h娜娜| 福利社在线免费观看| 湿好紧太硬了我太爽了| 日本人欧美xx| 99久久精品国产一区二区| 好大好硬好深好爽想要小雪| 亚洲国产精品久久久久久网站| 毛片的网站| 男人天堂中文字幕| 毛片免费毛片一级jjj毛片| 动漫美女羞羞视频| 爱情岛永久成人免费网站| 亚洲国产精品综合福利专区| 暖暖的视频完整视频韩国免费| 亚洲精品乱码久久久久久蜜桃| 放荡女小洁的性日记| 97导航| 99热com| 国产欧美日韩精品高清二区综合区 | 精品播放| 91色porny| freexxxx性大陆另类| 被调教的校花| 99久久这里只有精品| 国产专区视频在线观看| 我将她侵犯1~6樱花动漫在线看| 日本熟hdx|